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Proteomics, a powerful emerging technology for new biomarkers and drugs discovery, presents promising breakthroughs in diseases prevention and treatment. We apply proteomic techniques for search of disease biomarkers in the following: renal disease, liver cancer, necrotizing enterocolitis, diet-induced obesity as well as Chinese medicinal mushroom and toxic marine algae. |
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Representative
publications: |
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Ngai Heidi Hoi-Yee, Sit Wai Hung, Jiang Ping-Ping, Thongboonkerd Visith, Wan Jennifer Man Fan (2007). Differential Proteomics Study Reveals Urinary Preprohaptoglobin and Haptoglobin as Potentially Novel Biomarkers for Passive Heymann Nephritis. (In press, J Proteome Research). | ||
Ngai Heidi Hoi-Yee, Sit Wai Hung, Jiang Ping-Ping, Thongboonkerd Visith, Wan Jennifer Man Fan (2007). Differential Proteomics Study Reveals Urinary Preprohaptoglobin and Haptoglobin as Potentially Novel Biomarkers for Passive Heymann Nephritis. (In press, J Proteome Research). | ||
Cheuk-Lun Lee, Ping-Ping Jiang, Wai-Hung Sit and Jennifer Man-Fan Wan (2007) Proteome of Human T lymphocytes with Treatment of Cyclosporine and Polysaccharopeptide: Analysis of Significant Proteins that Manipulate T cells Proliferation and Immunosuppression. (In press, International Immunopharmacology. | ||
Ngai Heidi Hoi-Yee, Sit Wai Hung, Jiang Ping-Ping,
Xu Ruo-Jun, Wan Jennifer Man Fan,
Thongboonkerd Visith. Serial Changes in Urinary Proteome profile of
membranous nephropathy: implications for Pathophysiology and biomarker
discovery. J Proteome Research 2006,
5, 3038-3047. |
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Leo Lai Chan, Wai-Hung Sit, Paul Kwan-Sing Lam, Dennis Paul Hsientang Hsieh, Ivor John Hodgkiss, Jennifer Man-Fan Wan, Alvin Yam-Tat Ho, Nicola Man-chi Choi and David Dudgeon. (2007) Identification and characterization of “Biomarker of toxicity” from the proteome of Paralytic Shellfish toxin (PST)-producing dinoflagellates Alexandrium tamarense (Dinophycae). Proteomics. (In press). | ||
Chan
L.L., Hodgkiss I.J., Lam P.K.S., Wan J.M.F., Chou N.H., Lum J.H.K., Lo
M.G.Y., Mak A.S.C., Sit W.H.
and Lo S.C.L. (2005) Use of 2-DE to differentiate morphospecies of Alexandrium minutum, a paralytic shellfish
poisoning toxins (PST)-producing dinoflagellate of Harmful Algal Blooms.
Proteomics 5: 1580-1593. |
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Chan L.L., Hodgkiss I.J., Wan J.M.F., Lum J.H.K., Mak A.S.C., Sit W.H. and Lo S.C.L. (2004) Proteomic study of a model causative agent of harmful algal blooms, Prorocentrum triestinum II: The use of differentially expressed protein profiles under different growth phases and growth conditions for bloom prediction. Proteomics 4: 3214-3226. | ||
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(2) Chinese Medicine Chinese
herbal medicines have been used to treat cancer, infection, inflammation,
diabetes, heart disease etc for more than three thousand years in |
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2.1 Chinese medicine and Cancer | |
Lee L, Yang XT and Wan JMF. (2006) The culture duration affects the immunomodulatory and anticancer effect of polysaccharopeptide derived from Coriolus versicolor. Enzyme and microbial technology 38: 14-21. | |
Lau CS, Fanya Zeng, Chung-Chau Hon, Wai-Hung Sit, Ken Yan-Ching Chow, Raymond Kin-Hi Hui, Ivy Ka-Man Law, Victor Wai-Lap Ng, Frederick Chi-Ching Leung and Jennifer Man-Fan Wan. (2005) Molecular characterization of Coriolus versicolor PSP-induced apoptosis in human promyelotic leukemic HL-60 cells using cDNA microarray. International Journal of Oncology 26: 0-00. | |
Hui
K.P.Y., Sit W.H. and Wan J.M.F.
(2005) Induction of S phase cells arrest and caspase activation by polysaccharide
peptide (PSP) isolated from coriolus
versicolor enhanced the cell-cycle-dependent
activity and apoptotic cell death of Doxorubicin and Etoposide but not
Cytarabine in HL-60 cells. Oncology Reports. 14(1): 145-165. |
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Xiaotong Yang, Wai-Hung Sit, Daniel Kwong-On Chan, Jennifer Man-Fan Wan. (2005) The Cell Death Process of the Anticancer Agent Polysaccharide-peptide (PSP) in Human Promyelocytic Leukemic HL-60 Cells. Oncology Reports 13(6): 1201-1221. | |
Cheng
Le Le, Yang Xiao Tong, Mi Ke, Wan Jennifer Man Fan, Yang Qing Yao.
Colorimetric detection of total Triterpene contents in Fruiting Bodies
and spores of Ganoderma Lucidum
(Leyss.Ex Fr) Karst (2005). Mycosystema Vol: 24 p. 317-320. |
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Yang
Xiatong-Tong, Wan JMF, Ke MI
and Feng HQ. (2003) The quantification of (1,3)-b-Glucan in edible and
medicinal mushroom polysaccharides by using limulus G test. Mycosystema 22 (2): 296-302. |
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Pang ZJ, Chen Y, Zhou M and Wan J. (1999) The effect of polysaccharide krestin on nitric oxide production in mouse peritoneal macrophages. Medical Science Research 27(5): 299-302. | |
Wan JMF, Yang M and Sit WH. (1997) Mechanisms of Yun Zhi control of tumor cell proliferation. Chinese J. Integrated and Western Medicine. 3: 73-77. | |
Wan, JMF and WH. Sit. Mechanisms of Yun Zhi Control
of Tumor Cell Proliferation. p244-246 Adanced Research in PSP. International
Symposium on Chinese Medicine, The |
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Heidi H.Y. Ngai, W. H. Sit and Jennifer M.F. Wan. (2005) The nephroprotective effects of the Herbal Medicine Preparation, WH30+, on the chemical-induced acute and chronic renal failure in rats. The American Journal of Chinese Medicine 33(3): 491-500. | |
2.2 Chinese medicine, Infection and Immune Function | |
Cheuk-Lun Lee, Ping-Ping Jiang, Wai-Hung Sit and Jennifer Man-Fan Wan. (2006). The culture duration affects the immunomodulatory and anticancer effect of polysaccharopeptide derived from Coriolus versicolor. (In press, International Immunopharmacology 38: 14-21. | |
Jennifer Man-Fan Wan, Wai-Hung Sit, Cheuk-Lun Lee, Kelvin Hoi-Man Fu and Daniel Kwong-On Chan, Protection of Lethal Toxicity of Endotoxin by Salvia miltiorrhiza BUNGE is via Reduction in Tumor Necrosis Factor Alpha Release and Liver Injury (2006). International Immunopharmacolog 6: 750-758. | |
Kumar
D. Siava Sundara Kumar, Lau Chak Sing, SS, Wan Jennifer MF, Yang Dan, Hyde Kevin D. Immunomodulatory compounds
from Pestalotiopsis leucothes,
an endophytic ungus from Tripterygium wilfordii (2005). Life Sciences 78:147-156. |
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Lee
J. Wan JMF. Immunostimulatory Efficiency
of YunZhi and Quercetin, p151-152. International Symposium on Chinese Medicine,
The |
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2.3 Chinese Medicine and Arthrosclerosis | |
Zhou M, Chen Y, Liu S, Ding Z, Pang Z, and Wan J. (1998). Oxidative and malondialdehyde modification of low-density lipoprotein; a comparative study of binding and degradation by macrophages and endothelial cells. British Journal of Biochemical Science, 55: 192-198. | |
Pang ZJ, Zhou M, Chen Y, Wan J. (1998). L929 cell-conditioned medium reduces the impairment in plasma membrane fluidity of U937 and J774 cell lines treated with tert-butyl hydroperoxide. Medical Science Research 26(4): 241-243. | |
Zhou M, Chen Y, Wan J. (1997). The polysaccharide krestin prevents lipoperoxidative injury to experimentally atherosclerotic rabbits. Medical Science Research 25: 137-140. | |
Chen Y, Zhou M, Leu N, Liu SX, Pang Z, Wan J. (1997). The polysaccharide krestin prevents plaque formation in experimentally atherosclerotic rabbits. Medical Science Research 25(5): 297-300. | |
Shang X L, Zhou M, Wan J. (1997). Apoptosis in mouse peritoneal macrophages induced by oxidized low-density lipoprotein and prevention by the polysaccharide kreskin. Medical Science Research 25(6): 429-437. | |
Zhou M, Chen Y, Pang Z, Wan J. (1997). Preparation and characterization of two monoclonal antibodies against oxidatively modified low-density lipoprotein (O-LDL) and detection of O-LDL in serum in patients with coronary heart disease. Medical Science Research 25(7): 445-449. | |
Shang XL, Chen Y, Zhou M and Wan J. (1997). Protective effect of the polysaccaride krestin on inhibition of lipopolysaccharides-induced nitric oxide production in macrophages caused by oxidized low-density lipoprotein. Medical Science Research 25(8): 507-509. | |
Chen Y, Zhou M, Wan J. (1997). Oxidative and malondialdehde modification of low-density liporotein: a comparative study. British Journal of Biomedical Science 54: 159-165. | |
Liu SX, Zhou M, Chen Y, Wen WY, and Wan J. (1996).Injury to mitochondria of mouse peritoneal macrophages caused by oxidised low-density lipoprotein, and protection afforded by polysaccharide krestin. Medical Science Research 24(10): 715-717. | |
Pang
ZJ, Zhou M, Chen Y and Wan J. (1998). A Protein-bound Polysaccharide
Synergistic with Lipopolysaccharide Induces Nitric Oxide Release and Antioxidant
Enzyme Activities in Mouse Peritoneal Macrophages. American Journal of Chinese Medicine 26(2):
133-141. |
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Zhou M, Chen Y, Liu S, Ding Z, Pang Z, and Wan J. (1998). The effect of tert-butyl hydroperoxide on peritoneal macrophages and the protective effect of protein-bound polysaccharide administered intraperitoneally and orally. American Journal of Chinese Medicine 26(3-4): 301-310. |
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(3) Nutrition Research Nutrition is closely linked to health and disease. At the genetic level (nutrigenomics), certain nutrients can upset genes-balance leading to diseases such as cancer, obesity and diabetes etc. Significant nutrition research contributions are in the following areas: (i) Specific nutrients for hospital nutrition, (ii) oxidative stress and dietary antioxidant (ii) dietary lipids and cancer, (iii) dietary lipids and immune function; (iii) shock and burn; (iv) nutrition and cytokines etc. |
Representative
publications: |
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3.1. Nutrition and Cancer | |
Chiu
LCM, Ooi V, Wan
J MF. (2001) Eicosapentaenoic
acid modulates cyclin expression and arrests cell cycle progression I
human leukemic K-562 cells. International
Journal of Oncology
19: 845-849. |
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Chiu L, Ooi VE and Wan JMF (2000) Induction of apoptosis by dietary polyundsaturated fatty acids in human leukemic cells is not associated with DNA fragmentation. International Journal of Oncology 17: 789-796. | |
Chiu LCM and Wan JMF. (1999) Induction of apoptosis in HL-60 cells by eicosapentaenoic acid (EPA) is assoicated with downregulation of bcl-2 expression. Cancer Letters 145: 17-27. | |
Istfan NW, Wan J, Chen ZY. (1995) Fish Oil and cell proliferation kinetics in a mammary carcinoma tumor model. Advances in Experimental Medicine & Biology 375: 149-56. | |
Istfan N.W. Wan J.M. Bistrian B.R. and Chen, Z.Y. (1994) DNA replication time account for tumor growth variation induced by dietary fat in a breast carcinoma model. Cancer Letters 86(2): 177-86. | |
Istfan NW. Wan JM, Bistrian BR. (1993) Nutrition and tumor promotion: In vivo methods for measurement of cellular proliferation and protein metabolism. Journal of Parenter Enteral Nutrition 16(6 Suppl): S76-S82. | |
Wan JMF, Istfan NW, |
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3.2. Nutrition, Infection and Immune Function |
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Wan JMF, Haw MP, Blackburn GL. (1989) Overview: Nutrition, immune function and
inflammation: an overview. Proceedings of the Nutrition Society 48:315-335. |
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Wan JMF, Teo TC, Babayan VK, Blackburn GL. (1988) Invited Comment: Lipids and the development of immune dysfunction. Journal of Parenter Enteral Nutrition 12(6 Suppl): 43S-52S. | |
Wan JM, Grimble RF. (1987) Effect of dietary linoleate content on the metabolic response of rats to Escherichia coli endotoxin. Clinical Science 72:383-385. | |
Lee J and Wan JMF. (2000) Vitamin E supplementation improves cell-mediated immunity and oxidative stress of Asian Men and Women. Journal of Nutrition 130(12): 2923-27. | |
Chung-Yung Jetty Lee and Jennifer Man-Fan Wan. (2002) Immunoregulatory and Antioxidnat performance of a-tocopherol and selenium on human lymphocytes. Biological Trace Element Research 86(2): 123-135. | |
Wan JMF, Grimble R. (1998) Diurnal Factors Influence the Metabolic Response to Endotoxins. Nutrition Research 18: 265-272. | |
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Fogt F, Wan JM, O’Hara C, Bistrain BR, Blackburn GL, Istfan NW. (1991) Flow Cytometric measurement of cell kinetics in rat walker-256 carcinoma following in vivo and in vitro pulse labelling with bromodeoxyuridine. Cytometry 12:33-41. | |
Wan JM, Fogt F, Bistrian BR and Istfan NW. (1993) Evaluation of the tumoricidal effect of tumor necrosis factor in relationship to tumor protein dynamics and cell kinetics in Walker-256 carcinosarcoma bearing rat. American Journal of Physiology-Cell Physiology 265: 365-374. | |
Wan JM, Ye SH, Bistrian BR, and Isftan NW. (1994) The Effect of Interleukin-2 on Walker-256 tumor cell-cycle kinetics: -measured by flow cytometry. Journal of Leukocyte Biology 55(2): 241-247. | |
Wan, J. M., Istfan N.W., Ye, S-L and Bistrian B.R. (1995) Insulin-like growth factor- 1 is not mitogenic for the Walker-256 Carcinosarcoma. Life Sciences 56(10): 747-756. | |
Bagley JS, Wan J, Georgieff M, Forse RA, Blackburn GL. (1991) Cellular nutrition in support of early multiple organ failure. Chest 100:182S-188S. | |
Teo TC, Selleck KM, Wan JM, Pomposelli JJ, Babayan VK, Blackburn GL, Bistrian BR. (1991) Long term feeding with structured lipid composed of MCT and fish oil ameliorates endotoxic shock in guinea pigs. Metabolism 40(11): 1152-1159. | |
Pscheide EM, Wan JM, Blackburn GL, Bistrian BR, Istfan NW. (1992) Influence of w-3 fatty acids on splanchic blood flow and lactate metabolism in an endotoxemic rat model. Metabolism 41: 698-705. | |
Selleck, K, Wan J.M., Gollaher C. Babayan V, Bistrian B. (1994) Energy expenditure in gastrostomy-fed burned rats receiving variable amounts of dietary fat. American Journal of Clinical Nutrition 60: 216-222. | |
Research Links and Current Collaborators:
1. Professor
Per Sanglid, Department of Human Nutrition, Royal Veterinary and Agricultural
University, Denmark.
2. Professor M Wang, Chengdu University of Traditional Chinese Medicine,
Chengdu.
3. Professor ST Fan, Department of Surgery, The University of Hong Kong.
4. Professor CM, Che, Department of Chemistry, The University of Hong
Kong.
5. Dr. LL Chan, Department of Ecology and Biodiversity, The University
of Hong Kong.
6. Dr. Y. Sadovy, Department of Ecology and Biodiversity, The University
of Hong Kong.
7. Professor B Bistrain, Cancer Research Institute, Harvard Medical School,
USA. Departments of Medicine and Chemistry, The University of Hong Kong.
8. Dr. Yang Q Y. Department of Microbiology, Shanghai Teachers University,
Shanghai.
International/Special Recognition:
1. Invited
Plenary Lecture Speaker, The International Symposium on Bioinformatics and
ChineseMedicine, organized by the Chengdu TCM University, Chengdu, China in
2004; Topic – Anti-inflammatory effect of Denshen”.
2. Invited Plenary Lecture Speaker, The 10th World Congress on Advances
in Oncology and the 8th International Symposium on Molecular Medicine, Crete,
Greece, 2005; Topic - The anticancer mechanisms of Yun Zhi polysaccharide peptides.
3. Invited Symposium lecture Speaker and Chairperson: During the 2nd,
4th, 6th and 10th Annual General Scientific Meeting of the Hong Kong Society
of Flow Cytometry, Hong Kong in 1998, 2000, 2002, 2005; Topic areas: flow cytometry
technology, Chinese medicine and cancer.
4. Invited Symposium Lecture Speaker and Chairperson: International Symposium
on Chinese Medicine, Hong Kong in 1996, 1999; organized by The University of
Hong Kong and The Hong Kong Association for Health Care.
5. Invited Symposium Lecture Speaker: The Hong Kong Society of Immunology
2005, HK.
Organizing Conferences:
1. Organizing
committee member and co-chairperson on ” First International symposium in Chinese
Medicine” jointly organized by the University of Hong Kong, and Hong Kong Association
for Health Care Ltd. HK in 1996.
2. Organizing
committee member and co-chairperson on ” International Conference on Polysaccharide
peptide Research” organized by the Hong Kong Association for Health Care Ltd.
HK in 1996.
3. Organizing
committee member on “Hong Kong – the Centre for Chinese Medicine Development?”,
organized by the Hong Kong Society of Traditional Medicine and Natural Products
(1999).
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