Plant BIOCHEMISTRY LABORATORY

Principle Investigator: Clive Lo

 School of Biological Sciences

The University of Hong Kong

PUBLICATIONS

Lam LPY, Lui ACW, Bartley LE, Mikami B, Umezawa T*, Lo C* (2024) Multifunctional 5-hydroxyconiferaldehyde O-methyltransferases (CAldOMTs) in plant metabolism.  Journal of Experimental Botany DOI: 10.1093/jxb/erae011

Lui ACW, Pow KC, Lin N, Lam LPY, Liu Q, Godwin ID, Fan Z, Khoo CJ, Tobimatsu Y, Wang L, Hao Q*, Lo C* (2023) Regioselective stilbene O-methylations in Saccharinae grasses.  Nature Communications 14:3462.

Lam LPY, Wang L, Lui ACW, Liu H, Umezawa T, Tobimatsu Y, Lo C* (2023) Flavonoids in major cereal grains: distribution, functions, biosynthesis, and applications.  Phytochemistry Reviews.   22:1399-1438. 

Panthapulakkal Narayanan S, Alahakoon AY, Elliott CE, Russell D, Taylor PWJ, Lo C,  Chye ML (2023) Overexpression of rice acyl-CoA-binding protein OsACBP5 protects Brassica napus against seedling infection by fungal phytopathogens.  Crop & Pasture Science 74:459-469.

Lam PY, Wang L, Lo C, Zhu FY (2022) Alternative splicing and its roles in plant metabolism. International Journal of Molecular Sciences 23:7355.

Lam PY, Wang L, Lui ACW, Liu H, Takeda-Kimura Y, Chen MX, Zhu FY, Zhang J, Umezawa T, Tobimatsu Y, Lo C* (2022)   Deficiency in flavonoid biosynthesis genes CHS, CHI, and CHIL alters rice flavonoid and lignin profiles. Plant Physiology 28:1993-2011.

Liu H, Gong X, Deng H, Tan J, Sun Y, Wang F, Wu W, Zhou Z, Xu R, He H, Lo C (2022)  The rice aspartyl-tRNA synthetase YLC3 regulates amino acid homeostasis and chloroplast development under low temperature. Frontiers in Plant Sciences 13:847364.

Lam PY, Lui ACW, Wang L, Liu H, Umezawa T, Tobimatsu Y, Lo C* (2021) Tricin biosynthesis and bioengineering.  Frontiers in Plant Sciences 12:733198.

Liao P, Lung SC, Chan WL, Hu M, Kong GC, Bach TJ, Hao Q, Lo C, Chye ML (2021) Overexpression and inhibtion of 3-hydroxy-3-methylglutaryl-CoA synthase affect central metabolic pathways in tobacco.  Plant Cell Physiology 62:205-218.

Panthapulakkal Narayanan S, Lung SC, Liao P, Lo C, Chye ML (2020) The overexpression of OsACBP5 protects transgenic rice against necrotrophic, hemibiotrophic and biotrophic pathogens. Scientific Reports 10:14918.

Padmanabhan A, Tong Y, Wu Q, Lo C, Shah NP (2020) Proteomic analysis reveals potential factors associated with enhanced EPS production in Streptococcus thermophilus ASCC 1275. Scientific Reports 10:807.

Wang L, Lui, ACW, Lam PY, Liu, G, Godwin I, Lo C* (2020) Transgenic expression of flavanone 3-hydroxylase redirects flavonoid biosynthesis and alleviates anthracnose susceptibility in sorghum. Plant Biotechnology Journal 18:2170-2172.

Lui ACW, Lam PY, Chan KH, Wang L, Tobimatsu Y, Lo C* (2020) Convergent recruitment of 5'-hydroxylase activities by CYP75B flavonoid B-ring hydroxylases for tricin biosynthesis in Medicago legumes.  New Phytologist 228:269-284.  

Wang L, Lam PY, Lui ACW, Zhu FY, Chen MX, Liu H, Zhang J, Lo C* (2020) Flavonoids are indispensable for complete male fertility in rice.  Journal of Experimental Botany 71:4715-4728.

Liao P, Lung SC, Chan WL, Bach TJ, Lo C, Chye ML (2020) Overexpression of HMG-CoA synthase promotes Arabidopsis root growth and adversely affects glucosinolate biosynthesis.  Journal of Experimental Botany 71:272-289.

Wang L, Chen M, Zhu F, Fan T, Zhang J, Lo C* (2020) Alternative splicing is a Sorghum bicolor defense response to fungal infection.  Planta 251:14.  

Lam PY, Tobimatsu Y, Matsumoto N, Suzuki S, Lan W, Takeda Y, Yamamura M, Sakamoto M, Ralph J, Lo C, Umezawa T (2019) OsCAldOMT is a bifunctional O-methyltransferase involved in the biosynthesis of tricin-lignins in rice cell walls.  Scientific Reports 9:11597.

Lam PY, Lui ACW, Yamamura M, Wang L, Takeda Y, Suzuki S, Liu H, Zhu FY, Chen MX, Zhang J, Umezawa T, Tobimatsu Y, Lo C* (2019) Recruitment of specific flavonoid B-ring hydroxylases for two independent biosynthesis pathways of flavone-derived metabolites in grasses. New Phytologist 223:204-219.

Panthapulakkal Narayanan S, Liao P, Taylor PWJ, Lo C, Chye ML (2019) Overexpression of a monocot acyl-CoA-binding protein confers broad-spectrum pathogen protection in a dicot. Proteomics  19:e1800368.

Huang K, Ru B, Zhang Y, Chan WL, Chow SC, Zhang J, Lo C, Lui WY (2019) Sertoli cell-specific coxsackievirus and adenovirus receptor knockout regulates cell adhesion and gene transcription via β-catenin inactivation and Cdc42 activation. FASEB Journal 33:7588-7602.

Milne RJ, Dibley KE, Schnippenkoetter WH, Mascher M, Lui AC, Wang L, Lo C, Ashton AR, Ryan PR, Lagudah E (2019) The wheat Lr67 gene of the Sugar Transport Protein family confers multipathogen resistance in barley. Plant Physiology 179: 1285-1297.

Chen MX, Hu T, Zhu FY, Du ZY, Xue Y, Lo C, Chye ML (2018) Arabidopsis acyl-CoA-binding protein ACBP1 interacts with AREB1 and mediates salt and osmotic signaling in seed germination and seedling growth. Environmental and Experimental Botany. 156: 130-140.

Zhu FY, Chen MX, Chan WL, Yang F, Tian Y, Song T, Xie LJ, Zhou Y, Xiao S, Zhang J, Lo C* (2018) SWATH-MS quantitative proteomic investigation of nitrogen starvation in Arabidopsis reveals new aspects of plant nitrogen stress responses. Journal of Proteomics 187:161-170.

Schnippenkoetter W, Lo C, Liu G, Dibley K, Chan WL, White J, Milne R, Zwart A, Kwong E, Keller B, Godwin I, Krattinger S, Lagudah E (2017) The wheat Lr34 multi-pathogen resistance gene confers resistance to anthracnose and rust in sorghum. Plant Biotechnology Journal 15:1387-1396

Lam PY, Tobimatsu Y, Takeda Y, Suzuki S, Yamamura M, Umezawa T, Lo C* (2017) Disrupting  flavone synthase II alters lignin and improves biomass digestibility. Plant Physiology 174:972-985.

Zhu FY, Chen MX, Su YW, Xu X, Ye NH, Cao YY, Lin S, Liu TY, Li HX, Wang GQ, Jin Y, Gu YH, Chan WL, Lo C, Peng X, Zhu G, Zhang J. (2016) SWATH-MS Quantitative analysis of proteins in the rice inferior and superior spikelets during grain filling.  Frontiers in Plant Science 7:1926.

Liu H, Li Q, Yang F, Zhu FY, Sun Y, Tao Y, Lo C* (2016) Differential regulation of protochlorophyllide oxidoreductase abundances by VIRESCENT 5A (OsV5A) and VIRESCENT 5B (OsV5B) in rice seedlings.  Plant and Cell Physiology 57: 2392-2402.

Zhu FY, Chan WL, Chen MX, Kong RPW, Cai C, Wang Q, Zhang JH, Lo C* (2016) SWATH-MS quantitative proteomic investigation reveals a role of jasmonic acid during lead response in Arabidopsis.  Journal of Proteome Research 15: 3528-3539.

Liang C, Cheng S, Zhang Y, Sun Y, Fernie AR, Kang K, Panagiotou G, Lo C, Lim BL (2016) Transcriptomic, proteomic and metabolic changes in Arabidopsis thaliana leaves after the onset of illumination.  BMC Plant Biology 16:43.

Lam PY, Liu H, Lo C* (2015) Completion of tricin biosynthesis pathway in rice: Cytochrome P450 75B4 is a novel chrysoeriol 5'-hydroxylase.  Plant Physiology 168:1527-1536.

Zhu FY, Li L, Zhang J, Lo C* (2015) Transgenic expression of a sorghum gene (SbLRR2) encoding a simple extracellular leucine-rich protein enhances resistance against necrotrophic pathogens in Arabidopsis.  Physiological and Molecular Plant Pathology 91:31-37.

Li Q, Zhu FY, Gao X, Sun Y, Li S, Tao Y, Lo C*, Liu H (2014) Young Leaf Chlorosis 2 encodes the stromal-localized heme oxygenase 2 which is required for normal tetrapyrrole metabolism in rice.  Planta 240:701-712. 

Lam PY, Zhu FY, Chan WL, Liu H, Lo C* (2014) Cytochrome P40 93G1 is a flavone synthase II which channels flavanones to the biosynthesis of tricin O-linked conjugates in rice.  Plant Physiology 165:1315-1327.

Chen X, Chan WL, Zhu FY, Lo C* (2014) Phosphoproteomic analysis of the non-seed vascular plant model Selaginella moellendorffii.  Proteome Science 12:16.

Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Lo C, Chu I, Shi T, Huang J (2013) Proteomic evidence for genetic epistasis: clpr4 mutation switches leaf variegation to virescence in Arabidopsis.  The Plant Journal 76:943-956.

Li L, Zhu FY, Liu H, Chu A, Lo C (2013) Isolation and expression analysis of defense-related genes in sorghum-Colletotrichum sublineolum interaction.  Physiological and Molecular Plant Pathology 84:123-130.

Zhu FY, Li L, Lam PY, Chen MX, Chye ML, Lo C* (2013) Sorghum extracellular leucine-rich repeat protein SbLRR2 mediates lead tolerance in transgenic Arabidopsis.  Plant and Cell Physiology 54:1549-1559.

Wang Y, Lin YC, So J, Du Y, Lo C* (2013) Conserved metabolic steps for sporopollenin precursor formation in tobacco and rice.  Physiologia Plantarum 149:13-24.

Zhu Q, Qian Y, Zheng Z, Lo C, Chen F, Wang M (2013) Natural polyphenols alleviated lipid peroxidation-induced modification on BSA.  Journal of Functional Foods 5:355-361.

Lam MPY, Lau E, Siu SO, Ng DCM, Lo C, Chu IK (2011) Online combination of reverse-phase-reverse-phase and porous graphitic carbon liquid chromatography for multi-component separation of proteomics and glycoproteomics samples.  Electrophoresis 32:2930-2940.

Kong RPW, Siu SO, Lo C*, Chu IK (2011) Development of online high-/low-pH reverse-phase/reverse-phase two-dimensional liquid chromatography for shotgun proteomics: A reverse-phase-strong cation exchange-reverse-phase approach.  Journal of Chromatograph A 1218:3681-3688.

Lau E, Lam MPY, Siu SO, Kong RPW, Chan WL, Zhou Z, Huang J, Lo C*, Chu IK* (2011) Combinatorial use of offline SCX and online RP-RP liquid chromatography for iTRAQ-based quantitative proteomics applications.  Molecular Biosystems 7:1399-1408. 

Du Y, Chu H, Chu IK, Lo C* (2010) CYP93G2 is a flavanone 2-hydroxylase required for C-glycosyl-flavone biosynthesis in rice.  Plant Physiology 154:324-333. 

Liu H, Du Y, Chu H, Shih CH, Wong YW, Wang M, Chu IK, Tao Y, Lo C* (2010) Molecular dissection of the pathogen-inducible 3-deoxyanthocyanidin pathway in sorghum.  Plant and Cell Physiology 51:1173-1185.

Liu H, Lau E, Lam M, Chu H, Li S, Huang G, Guo P, Wang J, Jiang L, Chu IK, Lo C*, Tao Y* (2010) OsNOA1/RIF1 is a functional homolog of AtNOA1/RIF1: implication for a highly conserved plant cGTPase essential for chloroplast functions.  New Phytologist 187:83-105.

Zeng X, Kong R, Cheng, KW, Du Y, Tang YS, Chu IK, Lo C, Sze, KH, Chen, F, Wang M (2010) Direct Trapping of Acrylamide as a Key Mechanism for Niacin's Inhibitory Activity in Carcinogenic Acrylamide Formation.  Chemical Research in Toxicology 23:802-807.

Du Y, Chu H, Wang M, Chu IK, Lo C* (2010) Identification of flavone phytoalexins and a pathogen-inducible flavone synthase II gene in sorghum.  Journal of Experimental Botany 61:983-94.

Zeng XH, Cheng KW, Du Y, Kong R, Lo C, Chu IK, Chen F, Wang M (2010) Activities of hydrocolloids as inhibitors of acrylamide formation in model systems and fried potato strips.  Food Chemistry 121: 424-428.

Cheng KW, Yang RY, Tsou SCS, Lo C, Ho CT, Lee TC, Wang M (2009) Analysis of antioxidant activity and antioxidant constituents of Chinese toon.  Journal of Functional Foods 1: 253-259.

Cheng KW, Wong CC, Chao J, Lo C, Chen F, Chu IK, Ho CT, Wang M (2009) Inhibition of mutagenic PhIP formation by EGCG via scavenging of phenylacetaldehyde.  Molecular Nutrition & Food Research 53: 716-725.

Tang LK, Chu H, Yip WK, Yeung EC, Lo C* (2009) An anther specific dihydroflavonol 4-reductase-like gene (DRL1) is essential for male fertility in Arabidopsis.  New Phytologist 181: 576-587. (selected for cover image)

Lo C, Nicholson RL (2008) Phenolic compounds and their importance in plant diseases.  In: Leite B, Pascholati SF, Stangarlin JR, Cia P, eds, INTERAÇÃO PLANTA-PATÓGENO Fisiologia, Bioquímica e Biologia Molecular(Physiology, Biochemistry and Molecular Biology of Plant-Pathogen Interactions).  FEALQ: Piracicaba, Sao Paulo. pp. 285-303.  (Invited Book Chapter)

Shih CH, Chu H, Tang LK, Sakamoto W, Maekawa M, Chu IK, Wang M, Lo C* (2008) Functional characterization of key structural genes in rice flavonoid biosynthesis.  Planta 228: 1043-1054.

Cheng KW, Wong CC, Cho CK, Chu IK, Sze KH, Lo C, Chen F, Wang M (2008) Trapping of phenylacetaldehyde as a key mechanism responsible for naringenin's inhibitory activity in mutagenic 2-Amino-1-methyl-6-phenylimidazo [4,5-b] pyridine formation.  Chemical Research in Toxicology 21: 2026-2034.

Shih CH, Chen Y, Wang M, Chu IK, Lo C* (2008) Accumulation of isoflavone genistin in transgenic tomato plants over-expressing a soybean isoflavone synthase gene.  Journal of Agricultural and Food Chemistry 56: 5655-5661.

Peng C, Cheng KW, Ma J, Chen B, Ho CT, Lo C,  Chen F, Wang M (2008) Cinnamon bark proanthocyanidins as reactive carbonyl scavengers to prevent the formation of advanced glycation endproducts.  Journal of Agricultural and Food Chemistry 56: 1907-1911.

Yu CKY, Shih CH, Chu IK, Lo C (2008) Accumulation of trans-piceid in sorghum seedlings infected with Colletotrichum sublineolum.  Phytochemistry 69: 700-706. 

Lo C, Le Blanc Y, Yu CKY, Sze KH, Ng DCM, Chu IK (2007) Detection, characterization, and quantification of resveratrol glycosides in transgenic Arabidopsis over-expressing a sorghum stilbene synthase gene by liquid chromatography tandem mass spectrometry.  Rapid Communications in Mass Spectrometry 21: 4101-4108.

Shih CH, Siu SO, Ng R, Wong E, Chiu LCM, Chu IK, Lo C* (2007) Quantitative analysis of anticancer 3-deoxyanthocyanidins in infected sorghum seedlings.  Journal of Agricultural and Food Chemistry 55: 254-259.

Shih CH, Chu IK, Yip WK, Lo C* (2006) Differential expression of two flavonoid 3'-hydroxylase cDNAs involved in the biosynthesis of anthocyanin pigments and 3-deoxyanthocyanidin phytoalexins in sorghum.  Plant and Cell Physiology 47:1412-1419.

Yu CKY, Lam CNW, Springob K, Schmidt J, I Chu, Lo C (2006) Constitutive accumulation of cis-piceid in transgenic Arabidopsis over-expressing a sorghum stilbene synthase gene.  Plant and Cell Physiology 47:1017-1021.

Yu CKY, Springob K, Schmidt J, Nicholson RL, Chu IK, Yip WK, Lo C* (2005) A stilbene synthase gene is involved in host and non-host defense responses in Sorghum bicolor.  Plant Physiology 138: 393-401.

Lo C, Wang MN, Lam E (2005) Characterization of inducible gene silencing and recovery in transgenic plants with a vector designed for high-throughput analysis for gene functions. FEBS Letters 579:1498-1502.

Lo C, Adachi K, Shuster JR, Hamer JE, Hamer L (2003) The bacterial transposon Tn7 causes premature termination of mRNA in eukaryotic organisms: TAGKO mutagenesis in filamentous fungi.  Nucleic Acids Research 31: 4822-4827.

Lo C, Coolbaugh RC, Nicholson RL (2002) Molecular characterization and in silico expression analysis of a chalcone synthase gene family in sorghum.  Physiological and Molecular Plant Pathology 61: 179-188.

Lo C, Hamer L, Hamer JE (2002) Molecular characterization of a cystathionine b-synthase gene, CBS1, in Magnaporthe grisea. Eukaryotic Cell 1: 311-314.

Hamer L, Adachi K, Monetnegro-Chamorro MV, Tanzer M, Mahanty S, Lo C, Tarpey RW, Skalchunes, A, Heiniger R, Frank S, Darveaux B, Lampe DJ, Slater T, Lakshman R, DeZwaan T, Nelson G, Shuster J, Wossener J, Hamer JE.  (2001) Gene discovery and gene function assignment in industrial and pathogenic fungi. Proceedings of National Academy of Sciences USA 98: 5110-5115.

Lo C, Ho YS, Buswell JA (2001) Effect of phenolic monomers on laccase production by the edible mushroom, Pleurotus sajor-caju, and partial characterization of the major laccase component.  Mycologia 93: 413-421.

Lo C, de Verdier K, Nicholson RL (1999) Accumulation of 3-deoxyanthocyanidin phytoalexins and resistance to Colletotrichum sublineolum in sorghum. Physiological and Molecular Plant Pathology 55: 263-273.

Lo C, Hipskind JD, Nicholson RL (1999) cDNA cloning of a sorghum pathogenesis-related protein (PR-10) and differential expression of defense-related genes following inoculation with Cochiobolus heterostrophus or Colletotrichum sublineolum.  Molecular Plant-Microbe Interactions 12: 479-489.

Lo C, Nicholson RL (1998) Reduction of light-induced anthocyanin accumulation in inoculated sorghum mesocotyls: implications for a compensatory role in the defense response.  Plant Physiology 116: 979-989.

Lo C, Wood KV, Nicholson RL (1998) MALDI-TOF analysis of mixtures of 3-deoxyanthocyanidins and anthocyanins.  Phytochemistry 48: 1063-1066.

Lo C, Weiergang I, Bonham C, Hipskind J, Wood K, Nicholson RL (1996) Phytoalexin accumulation in sorghum: Identification of a methyl ether of luteolinidin.  Physiological and Molecular Plant Pathology 49: 21-31.