School of Biological Sciences

The University of Hong Kong

Professor Mee Len Chye

Emeritus Professor

BSc (Malaya); PhD (Melbourne)

HKU Scholars Hub https://hub.hku.hk/cris/rp/rp00687

ORCID https://orcid.org/0000-0003-3505-3674

mlchye@hku.hk

M.L. Chye, completed her PhD on a Commonwealth Scholarship at the University of Melbourne and received postdoctoral training in Plant Molecular Biology at the Rockefeller University (New York) and the Institute of Molecular and Cell Biology (Singapore). She joined the University of Hong Kong in 1993 and was appointed the Wilson and Amelia Wong Professor in Plant Biotechnology (2011-2024). She served as an Associate Dean (April 2010-April 2016) and Dean (May 2016-August 2019) of the Graduate School.

Listed as a top 1% cited scholar at HKU 2013-2022 (https://hub.hku.hk/local/top1pc/top1pc.jsp), her research awards include an Edward Clarence Dyason Universitas 21 Fellowship (2004/05), an Outstanding University Researcher Award (2006/07), a Croucher Senior Research Fellowship (2007/08), and an Eileen Mary Harris Scholarship (2013). She currently serves as an Associate Editor for Planta (Springer) and Plant Molecular Biology (Springer), and was Chair of the 4th Asian Symposium on Plant Lipids (2011) and the 12th International Symposium on Biocatalysis and Agricultural Biotechnology (ISBAB) in 2016. She has worked with international research collaborators from Australia, Canada, China, France, and the UK, as well as local collaborators in two AoEs (AoE/M-05/12 and AoE/M-403/16).

Her research focuses on elucidating the function of plant acyl-CoA-binding proteins (ACBPs) which bind and transport acyl-CoA esters. Her work revealed that plant ACBPs, classified into 4 classes in each of Arabidopsis, rice, Brassica napus and soybean, participate in development as well as stress responses arising from drought, salinity, low temperature and pathogens. Her team demonstrated that ACBPs work with protein partners in mediating development and stress tolerance, making ACBPs applicable for agriculture.

Her laboratory also identified the significance of the enzyme 3-hydroxy-3-methylglutaryl-CoA synthase (HMGS) in plant isoprenoid metabolism. In collaboration with Thomas Bach (CNRS Strasbourg), they reported that the expression of mutant and wild-type Brassica juncea HMGS in transgenic Arabidopsis upregulated the genes in the sterol biosynthetic pathway resulting in an overaccumulation of phytosterols (stimasterols, campesterol and stigmasterols). Furthermore, the overexpression of the HMGS variant S359A in tobacco, a model plant from the family Solanaceae, not only caused phytosterol accumulation but enhanced plant growth, pod size and seed yield. The overexpression of HMGS S359A in tomato produced fruits with increased phytosterols, squalene, provitamin A, lycopene and vitamin E (α-tocopherol), paving the way for biofortified food.

Her projects have been supported by the Wilson and Amelia Wong Endowment Fund, Research Grants Council of Hong Kong, NSFC/RGC Joint Research Scheme, “Centre for Organelle Biogenesis and Function” Area of Excellence AoE/M-05/12 (https://icell.sls.cuhk.edu.hk/research/aoe/), “Centre for Genomic Studies on Plant-Environment Interaction for Sustainable Agriculture and Food Security” Area of Excellence AoE/M-403/16” (https://aoegspei.cuhk.edu.hk/aboutus/) and the State Key Laboratory of Agrobiotechnology (CUHK).

HKU Press releases:

HKU scientists discover activation mechanisms in soybean for adaptation to saline soil in hope of improving agriculture productivity (12/1/22)
https://www.hku.hk/press/press-releases/detail/23924.html
The Croucher Foundation Newsletter (7/3/22)
https://projects.croucher.org.hk/news/hku-scientists-help-soybeans-to-grow-in-saline-soil

HKU plant scientists identify a new strategy to enhance rice grain yield (1/12/19)
https://www.hku.hk/press/press-releases/detail/20327.html
The Croucher Foundation Newsletter (31/12/19)
https://projects.croucher.org.hk/news/rice-protein-yields-hope-for-increased-food-supply-1?utm_source=Croucher+Foundation+Newsletter&utm_campaign=64de19ae2e-Newsletter+Issue+27+Jan+2018_COPY_01&utm_medium=email&utm_term=0_68f3b7ff76-64de19ae2e-142354273

HKU researchers generate tomatoes with enhanced antioxidant properties by genetic engineering (9/11/17)
https://www.hku.hk/press/news_detail_17055.html
Newsweek (14/11/17)
http://www.newsweek.com/genetic-engineering-antiaging-ageing-antioxidants-tomatoes-food-711299

Nikkei Asia Review (13/1/16)
http://asia.nikkei.com/Tech-Science/Science/Hong-Kong-scientists-engineer-plants-for-a-warmer-planet

HKU scientists discover a drought tolerance gene that may help plants survive global warming (22/11/15):
http://www.hku.hk/press/press-releases/detail/13590.html

HKU identifies a new strategy to protect flowers from freezing stress (9/6/14):
http://www.hku.hk/press/press-releases/detail/11311.html

Representative Publications

ZH Guo, TH Hu, M Fadhli Hamdan, M Li, R Wang, J Xu, SC Lung, W Liang, J Shi, D Zhang and ML Chye. 2024. A promoter polymorphism defines distinct roles in anther development for Col-0 and Ler-0 alleles of Arabidopsis ACYL-COA BINDING PROTEIN3. New Phytologist 243: 1424-1439 https://doi.org/10.1111/nph.19924
A Moradi, SC Lung and ML Chye. 2024. Interaction of soybean (Glycine max (L.) Merr.) Class II ACBPs with MPK2 and SAPK2 kinases: new insights into the regulatory mechanisms of plant ACBPs. Plants 13: 1146 https://doi.org/10.3390/plants13081146

S Panthapulakkal Narayanan, AY Alahakoon, CE Elliott, D Russell, PWJ Taylor, C Lo and ML Chye. 2023. Overexpression of rice acyl-CoA-binding protein OsACBP5 protects Brassica napus against seedling infection by fungal phytopathogens. Crop and Pasture Science https://doi.org/10.1071/CP22347

SC Lung, SH Lai, H Wang, X Zhang, A Liu, ZH Guo, HM Lam and ML Chye. 2022. Oxylipin signaling in salt-stressed soybean is modulated by ligand-dependent interaction of Class II acyl-CoA-binding proteins with lipoxygenase. Plant Cell 34:1117-1143. DOI: 10.1093/plcell/koab306
MF Hamdan, SC Lung, ZH Guo and ML Chye. 2022. Roles of acyl-CoA binding proteins in plant reproduction. J. Exp. Botany 73:2918-2936 DOI: 10.1093/jxb/erab499
AY Alahakoon, E Tongson, W Meng, ZW Ye, DA Russell, ML Chye, JF Golz and PWJ Taylor. 2022. Overexpressing Arabidopsis thaliana ACBP6 in transgenic rapid-cycling Brassica napus confers cold tolerance. Plant Methods 18:62. DOI: 10.1186/s13007-022-00886-y
P Liao, T Lechon, T Romsdahl, H Woodfield, S Fenyk, T Fawcett, E Wallington, RE Bates, ML Chye, KD Chapman, JL Harwood and S Scofield. 2022. Transgenic manipulation of triacylglycerol biosynthetic enzymes in B. napus alters lipid-associated gene expression and lipid metabolism. Sci. Rep. 12: 3352. https://doi.org/10.1038/s41598-022-07387-x
ZH Guo, SC Lung, MF Hamdan and ML Chye. 2022. Interactions between plant lipid-binding proteins and their ligands. Prog. Lipid Res. 86: 101156, https://www.sciencedirect.com/science/article/abs/pii/S016378272200011X

P Liao, SC Lung, WL Chan, M Hu, GKW Kong, TJ Bach, Q Hao, C Lo and ML Chye. 2021. Overexpression and inhibition of 3-hydroxy-3-methylglutaryl-CoA synthase affect central metabolic pathways in tobacco. Plant Cell Physiol. 62: 205-218. https://doi.org/10.1093/pcp/pcaa154
ZH Guo, G Pogancev, W Meng, ZY Du, P Liao, R Zhang and ML Chye. 2021. The overexpression of rice ACYL-COA-BINDING PROTEIN4 improves salinity tolerance in transgenic rice. Environ. Exp. Bot. 183: 104349-104363 https://doi.org/10.1016/j.envexpbot.2020.104349
A Liu, Z Xiao, Z Wang, HM Lam and ML Chye. 2021. Galactolipid and phospholipid profile and proteome alterations in soybean leaves at the onset of salt stress. Frontiers in Plant Science 12: 644408. https://doi.org/10.3389/fpls.2021.644408
NS Azlan, ZH Guo, WS Yung, Z Wang, HM Lam, SC Lung, ML Chye. 2021. In silico analysis of acyl-CoA-binding protein expression in soybean. Frontiers in Plant Science 12: 646938. https://doi.org/10.3389/fpls.2021.646938
SH Lai and ML Chye. 2021. Plant acyl-CoA-binding proteins - their lipid and protein interactors in abiotic and biotic stresses. Cells 10: 1064. https://doi.org/10.3390/cells10051064
ZH Guo and ML Chye. 2021. Investigations of lipid binding to acyl-CoA-binding proteins using isothermal titration calorimetry (ITC). Methods in Molecular Biology: Plant Lipids (Springer), edited by D Bartels and P Dörmann Chapter 23, pp. 401-415.

W Meng, L Xu, ZY Du, F Wang, R Zhang, X Song, SM Lam, G Shui, Y Li, and ML Chye. 2020. RICE ACYL-COA-BINDING PROTEIN6 affects acyl-CoA homeostasis and growth in rice. Rice 13: 75. https://thericejournal.springeropen.com/articles/10.1186/s12284-020-00435-y
S Panthapulakkal Narayanan, SC Lung, P Liao, C Lo and ML Chye. 2020. The overexpression of OsACBP5 protects transgenic rice against necrotrophic, hemibiotrophic and biotrophic pathogens. Sci. Rep. 10: 14918. https://doi.org/10.1038/s41598-020-71851-9
J Jin, ZH Guo, Q Hao and ML Chye. 2020. Crystal structure of the rice acyl-CoA-binding protein OsACBP2 in complex with C18:3-CoA reveals a novel binding pattern of binding to acyl-CoA esters. FEBS Letts. 594: 3568-3575. https://doi.org/10.1002/1873-3468.13923
JA Aznar-Moreno, M Venegas-Calerón, Z Du, R Garcés, JA Tanner, ML Chye, E Martínez-Force and JJ Salas. 2020. Characterization and function of a sunflower (Helianthus annuus L.) Class II acyl-CoA-binding protein. Plant Sci. 300: 110630 https://doi.org/10.1016/j.plantsci.2020.110630
P Liao, KP Leung, SC Lung, S Panthapulakkal Narayanan, L Jiang and ML Chye. 2020. Subcellular localization of rice acyl-CoA-binding proteins ACBP4 and ACBP5 supports their non-redundant roles in lipid metabolism. Front. Plant Sci. 11:331. https://doi.org/10.3389/fpls.2020.00331
N Amiruddin, PL Chan, N Azizi, PE Morris, KL Chan, PW Ong, R Rosli, SS Masura, DJ Murphy, R Sambanthamurthi, RP Haslam, ML Chye, JL Harwood and ETL Low. 2020. Characterisation of oil palm acyl-CoA-binding proteins and correlation of their gene expression with oil synthesis. Plant Cell Physiol. 61: 735-745. https://doi.org/10.1093/pcp/pcz237
Y Zhou, WJ Tan, LJ Xie, H Qi, YC Yang, LP Huang, YX Lai, YF Tan, DM Zhou, LJ Yu, QF Chen, ML Chye and S Xiao. 2020. Polyunsaturated linolenoyl‐CoA modulates ERF‐VII‐mediated hypoxia signaling in Arabidopsis. Journal of Integrative Plant Biology 62: 330-348, https://doi.org/10.1111/jipb.12875

ZH Guo, RP Haslam, LV Michaelson, EC Yeung, SC Lung, JA Napier and ML Chye. 2019. The overexpression of rice ACYL-COA-BINDING PROTEIN2 increases grain size and bran oil content in transgenic rice. Plant Journal 100: 1132-1147, https://doi.org/10.1111/tpj.14503
P Liao, HK Woodfield, JL Harwood, ML Chye and S Scofield. 2019. Comparative transcriptomics analysis of Brassica napus L. during seed maturation reveals dynamic changes in gene expression between embryos and seed coats and distinct expression profiles of acyl-CoA-binding proteins for lipid accumulation. Plant Cell Physiol. 60: 2812–2825, https://academic.oup.com/pcp/advance-article/doi/10.1093/pcp/pcz169/5555773
P Liao, SC Lung, W L Chan, TJ Bach, C Lo and ML Chye. 2019. Overexpression of HMG-CoA synthase promotes Arabidopsis root growth and adversely affects glucosinolate biosynthesis. J. Exp. Botany 71: 272-287, https://doi.org/10.1093/jxb/erz420
ZH Guo, ZW Ye, RP Haslam, LV Michaelson, JA Napier and ML Chye. 2019. Arabidopsis cytosolic acyl-CoA binding proteins function in determining seed oil content. Plant Direct 3: 1-9 e00182, DOI: 10.1002/pld3.182
SP Narayanan, P Liao, PWJ Taylor, C Lo and ML Chye. 2019. Overexpression of a monocot acyl-CoA-binding protein confers broad-spectrum pathogen protection in a dicot. Proteomics https://doi.wiley.com/10.1002/pmic.201800368
SC Lung, and ML Chye. 2019. Arabidopsis acyl-CoA-binding proteins regulate the synthesis of lipid signals. New Phytologist 223: 113-117, https://doi.org/10.1111/nph.15707
R Miao, SC. Lung, X Li, XD Li, and ML Chye. 2019. Thermodynamic insights into an interaction between ACYL-COA-BINDING PROTEIN2 and LYSOPHOSPHOLIPASE2 in Arabidopsis. J. Biol. Chem. 294: 6214-6226, https://www.jbc.org/content/early/2019/02/19/jbc.RA118.006876.full.pdf

MX Chen, TH Hu, FY Zhu, ZY Du, Y Xue, C Lo, ML Chye. 2018. Arabidopsis acyl-CoA-binding protein ACBP1 interacts with AREB1 and mediates salt and osmotic signaling in seed germination and seedling growth. Environ. Exp. Bot. 156: 130-140, DOI: 10.1016/j.envexpbot.2018.09.007
SC Lung, P Liao, E Yeung, AS Hsiao, Y Xue, and ML Chye. 2018. Arabidopsis ACYL-COA-BINDING PROTEIN1 interacts with STEROL C4-METHYL OXIDASE1-2 to modulate gene expression of homeodomain-leucine zipper IV transcription factors. New Phytologist 218: 183–200, DOI: 10.1111/nph.14965
TH Hu, SC Lung, ZW Ye and ML Chye. 2018. Depletion of Arabidopsis ACYL-COA-BINDING PROTEIN3 affects fatty acid composition in the phloem. Front. Plant Sci. 9:2. doi: 10.3389/fpls.2018.00002
P Liao, X Chen, M Wang, TJ Bach and ML Chye. 2018. Improved fruit α-tocopherol, carotenoid, squalene and phytosterol content through manipulation of Brassica juncea 3-HYDROXY-3-METHYLGLUTARYL-COA SYNTHASE1 in transgenic tomato. Plant Biotechnol. J. 16: 784–796, DOI: 10.1111/pbi.12828

SC Lung, P Liao, E Yeung, AS Hsiao, Y Xue, and ML Chye. 2017. Arabidopsis acyl-CoA-binding protein ACBP1 modulates sterol synthesis during embryogenesis. Plant Physiology 174: 1420-1435, DOI:10.1104/pp.17.00412
ZH Guo, WH. Chan, GK. Kong, Q Hao and ML Chye. 2017. The first plant acyl-CoA-binding protein structures: the close homologues OsACBP1 and OsACBP2 from rice. Acta Cryst. D73: 438-448, DOI:10.1107/S2059798317004193
ZW Ye, J Xu, J Shi, D Zhang and ML Chye. 2017. Kelch-motif containing acyl-CoA binding proteins AtACBP4 and AtACBP5 are differentially expressed and function in floral lipid metabolism. Plant Mol. Biol. 93: 209-225, DOI: 10.1007/s11103-016-0557-5
ZW Ye, QF Chen and M-L. Chye. 2017. Arabidopsis thaliana acyl-CoA-binding protein ACBP6 interacts with plasmodesmata-located protein PDLP8. Plant Signaling & Behaviour 12:8, e1359365, DOI: 10.1080/15592324.2017.1359365 http://dx.doi.org/10.1080/15592324.2017.1359365

ZY Du, T Arias, W Meng and ML Chye. 2016. Plant acyl-CoA-binding proteins: an emerging family in plant development and stress responses. Prog. Lipid Res 63: 165-181, DOI: 10.1016/j.plipres.2016.06.002
ZW Ye, SC Lung, TH Hu, QF Chen, YL Suen, M Wang, S Hoffmann-Benning, E Yeung and ML Chye. 2016. Arabidopsis acyl-CoA-binding protein ACBP6 localizes in the phloem and affects jasmonate composition. Plant Mol. Biol. 92: 717–730, DOI: 10.1007/s11103-016-0541-0.
Y Xue, SC Lung and ML Chye. 2016. Present status and future prospects of transgenic approaches for drought tolerance. In “Drought Tolerance in Plants, Vol 2: Molecular and Genetic Perspectives" (ISBN 978-3-319-32421-0) edited by LS Tran, DJ Burritt, MA Hossain, SH Wani and S Bhattacharjee. Springer, USA, Chapter 20, p. 549-569.
P Liao, A Hemmerlin, TJ Bach and ML Chye. 2016. The potential of the mevalonate pathway for enhanced isoprenoid production. Biotechnology Advances 34: 697-713.
SC Lung and ML Chye. 2016. Acyl-CoA-binding proteins in plant development. In “Lipids in plant and algae development” (ISBN 9783319259796) edited by Y Nakamura and Y Li-Beisson. Springer. Chapter 15, pp. 363-404. http://www.springer.com/us/book/9783319259772
JA Aznar-Moreno, M Venegas-Caleron, ZY Du, R Garces, JA Tanner, ML Chye, E Martinez-Force and JJ Salas. 2016. Characterization of a small acyl-CoA-binding protein (ACBP) from Helianthus annuus L. and its binding affinities. Plant Physiology and Biochemistry 102: 141-150.
SC Lung and ML Chye. 2016. Deciphering the roles of acyl-CoA-binding proteins in plant cells. Protoplasma 253: 1177-119, DOI 10.1007/s00709-015-0882-6.
SC Lung and ML Chye. 2016. The binding versatility of plant acyl-CoA-binding proteins and their significance in lipid metabolism. Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1861: 409-1421, Special Issue on Plant Lipid Biology 10.1016/j.bbalip.2015.12.018
ZW Ye and ML Chye. 2016. Plant cytosolic acyl-CoA-binding proteins. Lipids 51: 1-13, 50th Commemorative Issue DOI 10.1007/s11745-015-4103-z

ZY Du, MX Chen, QF Chen, JD Gu and ML Chye. 2015. Expression of Arabidopsis acyl-CoA-binding proteins AtACBP1 and AtACBP4 confers Pb(II) accumulation in Brassica juncea roots. Plant Cell Environ. 38: 101–117.
AS Hsiao, EC Yeung, ZW Ye and ML Chye. 2015. The Arabidopsis cytosolic acyl-CoA-binding proteins play combinatory roles in pollen development. Plant Cell Physiol. 56: 322-333.
YL Yung, MY Cheung, R Miao, YH Fong, KP Li, MH Yu, ML Chye, KB Wong and HM Lam. 2015. Site-directed mutagenesis shows the significance of interactions with phospholipids and the G-protein OsYchF1 on the physiological functions of the rice GTPase-Activating Protein 1 (OsGAP1). J. Biol. Chem. 290: 23984-23996.
YT Lai, YY Chang, L Hu, Y Yang, ZY Du, H Li, JA Tanner, ML Chye, C Qian and H Sun. 2015. Rapid labeling of intracellular His-tagged proteins in living cells. Proc. Natl Acad. Sci. USA 112: 2948-2953.

W Meng, AS Hsiao, C Gao, L Jiang and ML Chye. 2014. The subcellular localization of rice ACBPs indicates that OsACBP6-GFP is targeted to the peroxisome. New Phytologist 203: 469-482.
W Meng and ML Chye. 2014. Rice acyl-CoA-binding proteins OsACBP4 and OsACBP5 are differentially localized in the endoplasmic reticulum of transgenic Arabidopsis. Plant Signaling & Behavior 9:e29544.
P Liao, QF Chen and ML Chye. 2014. Transgenic Arabidopsis flowers overexpressing acyl-CoA-binding protein ACBP6 are freezing tolerant. Plant Cell Physiol. 55: 1055-1071.
P Liao, H Wang, M Wang, AS Hsiao, TJ Bach, ML Chye. 2014. Transgenic tobacco overexpressing Brassica juncea HMG-CoA synthase 1 shows increased plant growth, pod size and seed yield. PLOS ONE 9: e98264 (http://dx.plos.org/10.1371/journal.pone.0098264).
P Liao, H Wang, A Hemmerlin, DA Nagegowda, TJ Bach, M Wang and ML Chye. 2014. Past achievements, current status and future perspectives of studies on 3-hydroxy-3-methylglutaryl-CoA synthase (HMGS) in the mevalonate (MVA) pathway. Plant Cell Reports 33: 1005-1022.
MX Chen, SX Zheng, YN Yang,C Xu, Y Wang, JS Liu, WD Yang, ML Chye and HY Li. 2014. Strong seed-specific protein expression from the Vigna radiata storage protein 8SGα promoter in transgenic Arabidopsis seeds. Journal of Biotechnology 174: 49-56.
MX Chen, SC Lung, ZY Du and ML Chye. 2014. Engineering plants to tolerate abiotic stresses. In a special issue on "Transformation methods and innovation platforms in plant biotechnology". Biocatalysis and Agricultural Biotechnology 3: 81-87, edited by I. Kalvochuk and R. Weselake. Elsevier.
Y Xue, S Xiao, J Kim, SC Lung, L Chen, JA Tanner, MC Suh and ML Chye. 2014. Arabidopsis membrane-associated acyl-CoA-binding protein AtACBP1 is involved in stem cuticle formation. J. Exp. Bot. 18: 5473-5483.
AS Hsiao, RP Haslam. LV Michaelson. P Liao, JA Napier and ML Chye. 2014. Gene expression in plant lipid metabolism in Arabidopsis seedlings. PLOS ONE 9:e107372 (http://dx.plos.org/10.1371/journal.pone.0107372)
AS Hsiao, RP Haslam, LV Michaelson, P Liao, QF Chen, S Sooriyaarachchi, SL Mowbray, JA Napier, JA Tanner and ML Chye. 2014. Arabidopsis cytosolic acyl-CoA-binding proteins ACBP4, ACBP5 and ACBP6 have overlapping but distinct roles in seed development. Bioscience Reports 34: 865–877.

ZY Du, MX Chen, QF Chen, S Xiao and ML Chye. 2013a. Arabidopsis Acyl-CoA-Binding Protein ACBP1 participates in the regulation of seed germination and seedling development. Plant Journal 74: 294-309.
ZY Du, MX Chen, QF Chen, S Xiao and ML Chye. 2013b. Overexpression of Arabidopsis Acyl-CoA-Binding Protein ACBP2 enhances drought tolerance. Plant Cell Environ. 36: 300-314.
KW Kwan, ZW Ye, ML Chye and AHW Ngan. 2013. A mathematical model on water redistribution mechanism of the seismonastic movement of Mimosa pudica. Biophysical J. 105: 266-275
MX Chen, YN Yang, SX Zheng, C Xu, Y Wang, JS Liu, WD Yang, ML Chye and HY Li. 2013. A Vigna radiata 8S globulin α’ promoter drives efficient expression of GUS in Arabidopsis cotyledonary embryos. J. Agri. Food Chem. 61: 6423–6429.
ZY Du and ML Chye. 2013. Interactions between Arabidopsis acyl-CoA-binding proteins and protein partners. Planta 238: 239-245.
F Zhu, L Li, PY Lam, MX Chen, ML Chye and C Lo. 2013. Sorghum extracellular leucine-rich repeat protein SbLRR2 mediates lead tolerance in transgenic Arabidopsis. Plant Cell Physiol. 54: 1549-1559.

SX Zheng, S Xiao and ML Chye. 2012. The gene encoding Arabidopsis Acyl-CoA-Binding Protein 3 is pathogen-inducible and subject to circadian regulation. J. Exp. Bot. 63: 2985-3000.
H Wang, DA Nagegowda, R Rawat, P Bouvier-Navé, D Guo, TJ Bach and ML Chye. 2012. Overexpression of Brassica juncea wild-type and mutant HMG-CoA synthase 1 in Arabidopsis up-regulates genes in sterol biosynthesis and enhances sterol production and stress tolerance. Plant Biotechnol. J. 10: 31-42.

S Xiao and ML Chye. 2011a. Overexpression of Arabidopsis acyl-CoA-binding protein 3 enhances NPR1-dependent plant resistance to Pseudomonas syringae pv. tomato DC3000 Plant Physiology 156: 2069-2081.
S Xiao and ML Chye. 2011b. New roles for acyl-CoA-binding proteins in plant development, stress responses and lipid metabolism Prog. Lipid Res. 50: 141-151.
W Meng, YCF Su, RMK Saunders and ML Chye. 2011. The rice ACBP gene family: phylogeny, expression and functional analysis. New Phytologist 189: 1170-1184.

S Xiao, W Gao, QF Chen, SW Chan, SX Zheng, J Ma, M Wang, R Welti and ML Chye. 2010. Overexpression of Arabidopsis acyl-CoA-binding protein ACBP3 promotes starvation-induced and age-dependent leaf senescence. Plant Cell 22: 1463-1482.
W Gao, HY Li, S Xiao and ML Chye. 2010. Protein interactors of acyl-CoA-binding protein ACBP2 mediate cadmium tolerance in Arabidopsis. Plant Signaling & Behavior 5: 1025-1027.
S Xiao and ML Chye. 2010. The Arabidopsis thaliana ACBP3 regulates leaf senescence by modulating phospholipid metabolism and ATG8 stability. Autophagy 6: 802-804
W Gao, HY Li, S Xiao and ML Chye. 2010. Acyl-CoA-binding protein 2 binds lysophospholipase 2 and lysoPC to promote tolerance to cadmium-induced oxidative stress in transgenic Arabidopsis. Plant Journal 62: 989-1003.
ZY Du, S Xiao, QF Chen and ML Chye. 2010. Arabidopsis acyl-CoA-binding proteins ACBP1 and ACBP2 show different roles in freezing stress. Plant Signaling & Behavior 5: 607-609.
QF Chen, S Xiao, W Qi, G Mishra, J Ma, M Wang and ML Chye.2010. The Arabidopsis acbp1acbp2 double mutant lacking acyl-CoA-binding proteins ACBP1 and ACBP2 is embryo lethal. New Phytologist 186: 843-855.
Commentary
"As simple as ACB – new insights into the role of acyl-CoA-binding proteins in Arabidopsis" JA Napier and RP Haslam. New Phytologist 186: 781-783.
ZY Du, S Xiao, QF Chen and ML Chye.2010. Depletion of the membrane-associated acyl-CoA-binding protein ACBP1 confers freezing tolerance in Arabidopsis. Plant Physiology 152: 1585-1597.

W Gao, S Xiao, HY Li, SW Tsao and ML Chye. 2009. Arabidopsis thaliana acyl-CoA-binding protein ACBP2 interacts with a heavy-metal-binding farnesylated protein AtFP6. New Phytologist 181: 89-102.
HY Li and ML Chye. 2009. Use of green fluorescent protein to investigate expression of plant-derived vaccines. In “Viral applications of green fluorescent protein”, Methods in Molecular Biology 515: Chapter 19, pp. 275-287, edited by BA Hicks.
S Xiao and ML Chye. 2009. An Arabidopsis family of six acyl-CoA-binding proteins has three cytosolic members. Plant Physiol Biochem 47: 479-484.
S Xiao, QF Chen and ML Chye. 2009. Light-regulated Arabidopsis ACBP4 and ACBP5 encode cytosolic acyl-CoA-binding proteins that bind phosphatidylcholine and oleoyl-CoA ester. Plant Physiol Biochem 47: 926-933.
S Xiao, QF Chen and ML Chye. 2009. Expression of ACBP4 and ACBP5 proteins is modulated by light in Arabidopsis. Plant Signaling & Behavior 4: 1063-1065.
W Ubhayasekera, R Rawat, SWT Ho, M Wiweger, S Von Arnold, ML Chye and SL Mowbray. 2009. The first crystal structures of a family 19 class IV chitinase: the enzyme from Norway spruce. Plant Mol Biol 71: 277-289.

S Xiao, W Gao, QF Chen, S Ramalingam and ML Chye. 2008. Overexpression of membrane-associated acyl-CoA-binding protein ACBP1 enhances lead tolerance in Arabidopsis. Plant Journal 54: 141-151.
Y Guan, S Ramalingam, D Nagegowda, P Taylor and ML Chye. 2008. Brassica juncea chitinase BjCHI1 inhibits growth of fungal phytopathogens and agglutinates Gram-negative bacteria. J Exp Bot 59: 3475-3484.
QF Chen, S Xiao and ML Chye. 2008. Overexpression of the Arabidopsis 10-kilodalton acyl-CoA-binding protein ACBP6 enhances freezing tolerance. Plant Physiology 148: 304-315.
S Xiao and ML Chye. 2008. Arabidopsis ACBP1 overexpressors are Pb(II)-tolerant and accumulate Pb(II). Plant Signaling & Behavior 3: 693-695.
QF Chen, S Xiao and ML Chye. 2008. Arabidopsis ACBP6 is an acyl-CoA-binding protein associated with phospholipid metabolism. Plant Signaling & Behavior 3: 1019-1020.
S Xiao, HY Li, JP Zhang, SW Chan and ML Chye. 2008b. Arabidopsis acyl-CoA-binding proteins ACBP4 and ACBP5 are localized to the cytosol and ACBP4 depletion affects membrane lipid composition. Plant Mol Biol 68: 571-583.
HY Li, S Xiao and ML Chye. 2008. Ethylene- and pathogen-inducible Arabidopsis acyl-CoA-binding protein 4 interacts with an ethylene-responsive element binding protein. J Exp Bot 59: 3997-4006.
Y Guan and ML Chye.2008. A Brassica juncea chitinase with two-chitin binding domains shows anti-microbial properties against phytopathogens and Gram-negative bacteria. Plant Signaling & Behavior 3: 1103-1105.

W Ubhayasekara, CM Tang, SWT Ho, G Berlund, T Bergfors, ML Chye and SL Mowbray. 2007. Crystal structures of a family 19 chitinase from Brassica juncea show flexibility of binding cleft loops. FEBS J 274: 3695-3703.
R Welti, J Shah, W Li, M Li, J Chen, JJ Burke, ML Fauconnier, K Chapman, ML Chye and X Wang. 2007. Plant lipidomics: discerning biological function by profiling plant complex lipids using mass spectrometry. Frontiers in Bioscience 12: 2494-2506.
W Ubhayasekara, CM Tang, R Rawat, SWT Ho, ML Chye and SL Mowbray. 2007. Involvement of loops in catalysis in family 19 chitinases. Advances in Chitin Science 10: 147-152.
KC Leung, HY Li, S Xiao, MH Tse, ML Chye. 2006. Arabidopsis ACBP3 is an extracellularly targeted acyl-CoA-binding protein. Planta 223: 871-881.

SF Sin, EC Yeung, ML Chye. 2006. Down-regulation of Solanum americanum genes encoding proteinase inhibitor II causes defective seed development. Plant Journal 46: 58-70.
Y Zhou, MYT Lee, JMH Ng, ML Chye, WK Yip, SY Zee and E Lam. 2006. A truncated hepatitis E virus ORF2 protein expressed in tobacco plastids is antigenic in mice. World Journal of Gastroenterology 12: 306-312.
MYT Lee, Y Zhou, AH Chu, RWM Lung, ML Chye, WK Yip, SY Zee and E Lam. 2006. Expression of viral capsid protein antigen against Epstein-Barr virus in plastids of Nicotiana tabacum cv. SR1. Biotech Bioeng 94: 1129-1137.
HY Li, S Ramalingam and ML Chye. 2006. Accumulation of recombinant SARS-CoV spike protein in plant cytosol and chloroplasts indicate potential development of plant-derived vaccines. Expt Biol Medicine 231: 1346-1352.
F Pojer, JL Ferrer, SB Richard, DA Nagegowda, ML Chye, TJ Bach and JP Noel. 2006. Structural basis for the design of potent and species specific inhibitors of 3-hydroxy-3-methylglutaryl-coenzyme A synthases. Proc Natl Acad Sci USA 103: 11491-11496.

ML Chye, KJ Zhao, ZM He, S Ramalingam and KL Fung. 2005. An agglutinating chitinase BjCHI1 with two chitin-binding domains confers fungal protection in transgenic potato. Planta 220: 717-730.
D Nagegowda, S Ramalingam, A Hemmerlin, TJ Bach and ML Chye. 2005. Brassica juncea HMG-CoA synthase: localization of mRNA and protein. Planta 221: 844-856.
R Rawat, ZF Xu, KM Yao and ML Chye. 2005. Identification of cis-elements in ethylene and circadian regulation of gene encoding Solanum melongena cysteine proteinase. Plant Mol Biol 57: 629-643.

ZF Xu, WL Teng and ML Chye. 2004. Inhibition of endogenous trypsin- and chymotrypsin-like activities in transgenic lettuce expressing heterogeneous proteinase inhibitor SaPIN2a. Planta 218: 623-629.
HY Li and ML Chye. 2004. Arabidopsis acyl-CoA-binding protein ACBP2 interacts with an ethylene-responsive element binding protein AtEBP via its ankyrin repeats. Plant Mol Biol 54: 233-243.
KC Leung, HY Li, G Mishra and ML Chye. 2004. ACBP4 and ACBP5, novel Arabidopsis acyl-CoA-binding proteins with kelch motifs that bind oleoyl-CoA. Plant Mol Biol 55: 297-309.
SF Sin and ML Chye. 2004. Expression of proteinase inhibitor II proteins during floral development in Solanum americanum flowers. Planta 219: 1010-1022.
D Nagegowda, TJ Bach and ML Chye. 2004. Brassica juncea HMG-CoA synthase 1: expression and characterization of recombinant wild-type and mutant enzymes. Biochem J 383: 517-527.
CM Tang, ML Chye, S Ramalingam, SW Ouyang, KJ Zhao, W Ubhayasekera and S Mowbray. 2004. Functional analysis of the chitin-binding domains and the catalytic domain of Brassica juncea chitinase BjCHI1. Plant Mol Biol 56: 285-298.

ZF Xu, ML Chye, HY Li, FX Xu and KM Yao. 2003. G-box binding coincides with increased S. melongena cysteine proteinase expression in senescent fruits and circadian-regulated leaves. Plant Mol Biol 51: 9-19.
HY Li and ML Chye. 2003. Membrane localization of Arabidopsis acyl-CoA-binding protein ACBP2. Plant Mol Biol 51: 483-492.

KL Fung, KJ Zhao, ZM He and ML Chye. 2002. Tobacco-expressed Brassica juncea chitinase BjCHI1 shows antifungal activity in vitro. Plant Mol Biol 50: 283-294.

ZF Xu, WQ Qi, XZ Ouyang, E Yeung and ML Chye. 2001. A proteinase inhibitor II of Solanum americanum is expressed in phloem. Plant Mol Biol 47: 727-738.

D Alex, TJ Bach and ML Chye. 2000. Expression of Brassica juncea 3-hydroxy-3-methylglutaryl-CoA synthase is developmentally regulated and stress-responsive. Plant Journal 22: 415-426.
ML Chye, HY Li and MH Yung. 2000. Single amino acids substitutions at the acyl-CoA-binding domain interrupt 14[C]palmitoyl-CoA binding of ACBP2, an Arabidopsis acyl-CoA-binding protein with ankyrin repeats. Plant Mol Biol: 44: 711-721.

KJ Zhao and ML Chye. 1999. Methyl jasmonate induces expression of a Brassica juncea chitinase with two chitin-binding domains. Plant Mol Biol 40: 1009-1018.
ML Chye, BQ Huang and SY Zee. 1999. Isolation of a gene encoding Arabidopsis membrane-associated acyl-CoA binding protein and immunolocalization of its gene product. Plant Journal 18: 205-214.
FX Xu and ML Chye. 1999. Expression of cysteine proteinase during developmental events associated with programmed cell death in brinjal. Plant Journal 17: 321-327.

Last modified: 27/12/2018

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